LOCUS       40924_5239       11904 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB4cCGGW DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 11904)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..11904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        872..1217
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1238..1435
                     /label=cecfp
                     /note="cecfp"
     protein_bind    1445..1569
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1622..1652
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1706..2362
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHSCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2707..3009
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3053..3177)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3203..3455
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3489..3505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3513..3529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3537..3567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3582..3603)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3637..3889)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4282..5070)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5094..5277)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5483..5507)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6035..6823
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7070..7658
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7844..7984)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8328..8522)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8591..9655)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10092..10718)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      361 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      421 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      481 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      541 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      601 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      661 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      721 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      781 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      841 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      901 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      961 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1021 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1081 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1141 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1201 ttcatttgga gagaacacgg gggactctag aatgagcatg ggcagcgtgc agctcgccga
     1261 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1321 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1381 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggctgt
     1441 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1501 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1561 gtcactatgg cggcccctcg aggcctcgag ccctcgaggg gccgcattag gcaccccagg
     1621 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
     1681 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     1741 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     1801 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     1861 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     1921 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc actcttgtta
     1981 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     2041 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     2101 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     2161 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     2221 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     2281 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     2341 cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca
     2401 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     2461 aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     2521 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
     2581 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
     2641 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact
     2701 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     2761 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
     2821 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     2881 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     2941 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     3001 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
     3061 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     3121 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat
     3181 aacctagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3241 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3301 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3361 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3421 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg
     3481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3601 ttaggctgaa ttaggcgcgc ctatttctga attcccgatc tagtaacata gatgacaccg
     3661 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
     3721 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
     3781 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     3841 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggatcatcc
     3901 gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct
     3961 cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg gacgccgggc ccgatcatat
     4021 tgtcgctcag gatcgtggcg ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac
     4081 cttcgaccgc ctgttccgca gagatcccgt gggcgaagaa ctccagcatg agatccccgc
     4141 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     4201 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     4261 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     4321 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     4381 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     4441 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     4501 ggcatcgcca tgggtcacga cgagatcatc gccgtcgggc atgcgcgcct tgagcctggc
     4561 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     4621 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     4681 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     4741 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     4801 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     4861 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     4921 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4981 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     5041 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatccagatc cggtgcagat
     5101 tatttggatt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg
     5161 tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt
     5221 gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg
     5281 gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     5341 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tgaattcaga
     5401 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     5461 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     5521 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     5581 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     5641 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     5701 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     5761 cagtggacat aagcctcgtt cggttcgtaa gctgtaatgc aagtagcgta actgccgtca
     5821 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     5881 atggcttctt gttatgacat gtttttttgg ggtacagtct atgcctcggg catccaagca
     5941 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     6001 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     6061 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     6121 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     6181 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     6241 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     6301 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     6361 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     6421 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     6481 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     6541 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     6601 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     6661 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     6721 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     6781 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     6841 ttcgttcaag ccgacgccgc ttcgccggcg ttaactcaag cgattagatg cactaagcac
     6901 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     6961 aataagccct acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt
     7021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7201 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7861 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7921 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7981 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8041 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8101 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8161 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8221 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     8281 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     8341 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     8401 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     8461 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     8521 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     8581 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     8641 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     8701 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     8761 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     8821 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     8881 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     8941 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9001 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9061 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9121 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9181 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9241 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     9301 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     9361 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     9421 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     9481 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     9541 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     9601 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac
     9661 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
     9721 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
     9781 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
     9841 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
     9901 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
     9961 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    10021 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    10081 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    10141 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    10201 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    10261 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    10321 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    10381 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    10441 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    10501 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    10561 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    10621 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    10681 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    10741 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    10801 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    10861 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    10921 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    10981 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    11041 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    11101 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    11161 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    11221 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    11281 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    11341 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    11401 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    11461 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    11521 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    11581 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    11641 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    11701 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    11761 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    11821 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    11881 gcggaggggt tggatcaaag tact
//
